Composite

Part:BBa_K2525000:Design

Designed by: Abhilash Patel   Group: iGEM17_IIT_Delhi   (2017-10-27)


pOrf2-RBS-GFP-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 41
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 726


Design Notes

Part was biobrick rfc 10 compatible and therefore no significant issues.


Source

It is derived from Streptomyces griseus. The sequences are obtained from Addgene and synthesized with IDT.

References